快速蛋白质液相色谱法在尿液和脑脊液中血浆蛋白分离中的应用。

Applications of fast protein liquid chromatography TM in the separation of plasma proteins in urine and cerebrospinal fluid.

作者信息

Cooper E H, Turner R, Johns E A, Lindblom H, Britton V J

出版信息

Clin Chem. 1983 Sep;29(9):1635-40.

Abstract

Fast Protein Liquid Chromatography TM (FPLC), in which an anion-exchange column is used, provides rapid separation and reproducible profiling of the plasma proteins in urine and cerebrospinal fluid (CSF). Chromatographic separation of the proteins takes 1 h for urine specimens and 45 min for CSF. The elution sequence from the anion-exchange column is similar to the electrophoretic mobility. Individual proteins have the same retention times independently of which type of specimen is used. The elution characteristics of 21 plasma proteins have been identified. We illustrated some applications of this system, including the profiling of tubular protein-uria, the isolation of Bence Jones proteins from urine, and the investigation of hemoglobin-derived products in the CSF.

摘要

快速蛋白质液相色谱法（FPLC）使用阴离子交换柱，可快速分离尿液和脑脊液（CSF）中的血浆蛋白，并提供可重复的分析图谱。尿液标本的蛋白质色谱分离需要1小时，脑脊液则需要45分钟。从阴离子交换柱的洗脱顺序与电泳迁移率相似。无论使用何种类型的标本，单个蛋白质都具有相同的保留时间。已确定了21种血浆蛋白的洗脱特性。我们展示了该系统的一些应用，包括肾小管蛋白尿的分析、从尿液中分离本-周蛋白以及脑脊液中血红蛋白衍生产物的研究。