Sterin A B, Goldraij A, Gimeno M A, Gimeno A L
Eur J Pharmacol. 1983 Jun 17;90(4):411-7. doi: 10.1016/0014-2999(83)90563-0.
The experiments concerned the effects of 15 days of restricted diet (50% of the normal food intake) on the magnitude of phasic contractions (Isometric Developed Tension, IDT) of uterine horns isolated from estrous rats. During 60 min following isolation, the IDT of normally fed controls diminished significantly whereas preparations from animals on the restricted diet had a better contractile constancy. This improved motility was prevented by blockers of prostaglandin (PG) synthesis as well as by return to a normal diet. Cumulative dose-response curves for isoproterenol, norepinephrine and methoxamine were made for the different groups. Isoproterenol and norepinephrine evoked a dose-dependent inhibition of IDT. This response was significantly shifted to the right with uteri from restricted diet animals. Following incubation with propranolol, norepinephrine stimulated the magnitude of contractions significantly more in uterine horns from restricted diet rats than in those from controls. The dose-response curve showing the enhancement of motility induced by methoxamine was displaced to the left in preparations from animals on a restricted diet. This shift was abolished by restoring the normal diet as well as by the presence in vitro of indomethacin or acetylsalicylic acid. The results suggest that tissue PGS, the prevalence of alpha-adrenoceptor mechanisms and the reduction of beta-ones, are involved in the contractile behavior of the uterus isolated from restricted diet rats.
这些实验关注了15天限制饮食（正常食物摄入量的50%）对从发情期大鼠分离出的子宫角的相性收缩幅度（等长收缩张力，IDT）的影响。在分离后的60分钟内，正常喂食对照组的IDT显著降低，而限制饮食动物的子宫角制剂的收缩稳定性更好。前列腺素（PG）合成阻滞剂以及恢复正常饮食可阻止这种改善的运动性。为不同组制作了异丙肾上腺素、去甲肾上腺素和甲氧明的累积剂量-反应曲线。异丙肾上腺素和去甲肾上腺素引起IDT的剂量依赖性抑制。对于限制饮食动物的子宫，这种反应显著右移。与普萘洛尔孵育后，去甲肾上腺素对限制饮食大鼠子宫角收缩幅度的刺激明显大于对照组。显示甲氧明诱导运动性增强的剂量-反应曲线在限制饮食动物的制剂中向左移位。恢复正常饮食以及在体外存在吲哚美辛或乙酰水杨酸可消除这种移位。结果表明，组织PGS、α-肾上腺素能受体机制的优势以及β-肾上腺素能受体机制的减少，参与了从限制饮食大鼠分离出的子宫的收缩行为。
