Rapoport S, Dubiel W, Müller M
FEBS Lett. 1983 Aug 22;160(1-2):134-6. doi: 10.1016/0014-5793(83)80952-1.
The ATP-dependent proteolysis of rat liver mitochondria prepared in electrolyte-poor sucrose media requires the presence of Ca2+. Lanthanum, an inhibitor of Ca2+ uptake, inhibits the proteolysis. In contrast, proteolysis of mitochondria prepared in a salt medium does not require Ca2+, nor is it inhibited by lanthanum. It is concluded that Caa+ exerts its effect in an indirect manner, by causing swelling and thereby increasing the accessibility of the membrane proteins of the inner mitochondrial membrane.
在贫电解质蔗糖培养基中制备的大鼠肝脏线粒体的ATP依赖性蛋白水解需要Ca2+的存在。镧是一种Ca2+摄取抑制剂，可抑制蛋白水解。相比之下，在盐培养基中制备的线粒体的蛋白水解不需要Ca2+，也不受镧的抑制。得出的结论是，Ca2+通过引起肿胀从而增加线粒体内膜膜蛋白的可及性以间接方式发挥其作用。
