三氟拉嗪与猪钙调蛋白的相互作用：19F核磁共振和诱导圆二色光谱研究

Interaction of trifluoperazine with porcine calmodulin 19F NMR and induced CD spectral studies.

作者信息

Shimizu T, Hatano M

出版信息

FEBS Lett. 1983 Aug 22;160(1-2):182-6. doi: 10.1016/0014-5793(83)80962-4.

Abstract

We have used 19F NMR and CD spectra to study interactions of TFP with CaM under various conditions where no aggregation of TFP occurs. It was found that TFP is bound to CaM in the absence of Ca2+ with Ka greater than 4 X 10(4) M in terms of 19F NMR of TFP and that KCl markedly influences the interaction of TFP with CaM. It was also found that TFP causes quite strong induced CD bands corresponding with the absorption bands of the aromatic ring of TFP when TFP is bound to Ca2+-CaM. The induction of the CD bands was time-dependent and was composed of fast and slow phases.

摘要

我们利用19F核磁共振和圆二色光谱研究了在三氟拉嗪（TFP）不发生聚集的各种条件下TFP与钙调蛋白（CaM）的相互作用。结果发现，在没有Ca2+的情况下，TFP与CaM结合，就TFP的19F核磁共振而言，其结合常数Ka大于4×10(4) M，并且KCl显著影响TFP与CaM的相互作用。还发现，当TFP与Ca2+-CaM结合时，TFP会引起与TFP芳香环吸收带相对应的相当强的诱导圆二色带。圆二色带的诱导是时间依赖性的，由快速和慢速阶段组成。