Tanirbergenov T B, Igoshina A B, Tarasov V A
Genetika. 1983 Jun;19(6):888-96.
The lethal action of mitomycin C and the effect of mutual treatment with mitomycin C and spermidine on Escherichia coli were studied. DNA repair in cells treated with mitomycin C was shown to have some differences, as compared to that of UV-induced pyrimidine dimers. The presence of the additive sbcB mutation increases the resistance of wild-type bacteria as well as of recBrecC and recF mutants to the lethal action of mytomicin C. Preliminary treatment of bacteria with spermidine increases resistance to the lethal action of the mutagen in wild-type bacteria as well as uvrB, recBrecC and sbcB strains. However, no such effect was observed in recF, recFsbcB and uvrE strains. The data suggest that the protective action of spermidine may be connected with stimulation of RecF-pathway of postreplication repair.
研究了丝裂霉素C的致死作用以及丝裂霉素C与亚精胺联合处理对大肠杆菌的影响。与紫外线诱导的嘧啶二聚体相比，经丝裂霉素C处理的细胞中的DNA修复表现出一些差异。添加sbcB突变会增加野生型细菌以及recBrecC和recF突变体对丝裂霉素C致死作用的抗性。用亚精胺对细菌进行预处理可增加野生型细菌以及uvrB、recBrecC和sbcB菌株对诱变剂致死作用的抗性。然而，在recF、recFsbcB和uvrE菌株中未观察到这种效果。数据表明，亚精胺的保护作用可能与刺激复制后修复的RecF途径有关。
