肌醇磷脂代谢与成肌细胞融合
Inositol phospholipid metabolism and myoblast fusion.
作者信息
Wakelam M J
出版信息
Biochem J. 1983 Jul 15;214(1):77-82. doi: 10.1042/bj2140077.
Abstract
The fusion of chick embryonic myoblasts has been studied in tissue culture. Myoblasts are maintained at 0.1 microM-Ca2+ for 50 h. During this time they achieve fusion competence. Fusion is initiated by raising the medium Ca2+ concentration to 1.4 mM. A rapid breakdown of the polyphosphoinositides was detected within 3 min of Ca2+ addition. Rapid synthesis of phosphatidic acid was also detected at this time. Breakdown of phosphatidylinositol and synthesis of 1,2-diacylglycerol were also detected. Other phospholipids were unaffected. Sr2+ could replace Ca2+ in this process but Mg2+ could not and also inhibited the Ca2+ effect. The Ca2+-ionophore A23187 stimulated further apparent polyphosphoinositide breakdown in the presence of Ca2+. 6. The results are discussed with respect to myoblast fusion.
摘要
已在组织培养中对鸡胚成肌细胞的融合进行了研究。将成肌细胞在0.1微摩尔/升钙离子浓度下维持50小时。在此期间，它们获得融合能力。通过将培养基钙离子浓度提高到1.4毫摩尔/升来启动融合。在添加钙离子后3分钟内检测到多磷酸肌醇迅速分解。此时还检测到磷脂酸的快速合成。也检测到磷脂酰肌醇的分解和1,2 - 二酰基甘油的合成。其他磷脂未受影响。在这个过程中锶离子可以替代钙离子，但镁离子不能，而且还会抑制钙离子的作用。钙离子载体A23187在有钙离子存在的情况下会刺激进一步明显的多磷酸肌醇分解。6. 就成肌细胞融合对结果进行了讨论。
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本文引用的文献
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