Kujawa M J, Tepperman K
Dev Biol. 1983 Oct;99(2):277-86. doi: 10.1016/0012-1606(83)90277-4.
The effects of different glycosaminoglycans (GAGs) on myogenesis were tested by culturing embryonic chick myoblasts on tissue culture dishes to which either hyaluronic acid (HA) or chondroitin sulfate (ChS) was covalently bound. Both in cell number and in apparent cell type distribution, the population of cells bound to GAGs is similar to that on gelatin and significantly different from that observed with uncoated dishes. When plated on ChS, myoblasts proliferate, align, and fuse at a rate similar to cells plated on gelatin. The final fused cells appear as sheets rather than long, thin myotubes. On HA, the cells proliferate but are inhibited from differentiation. The extent of inhibition is dependent on the amount of HA present. The inhibition of myogenesis is maintained through four subcultures on HA, but can be reversed at any time by culturing cells on gelatin. These experiments indicate that different GAGs have different effects on myogenesis and that HA can actively inhibit the process.
通过在共价结合了透明质酸（HA）或硫酸软骨素（ChS）的组织培养皿上培养鸡胚成肌细胞，测试了不同糖胺聚糖（GAGs）对肌生成的影响。无论是细胞数量还是明显的细胞类型分布，与GAGs结合的细胞群体都与明胶上的相似，且与未包被培养皿上观察到的显著不同。当接种在ChS上时，成肌细胞以与接种在明胶上的细胞相似的速率增殖、排列并融合。最终融合的细胞呈现为片状而非长而细的肌管。在HA上，细胞增殖但分化受到抑制。抑制程度取决于HA的存在量。在HA上进行四次传代培养后，肌生成的抑制作用仍然存在，但通过在明胶上培养细胞，这种抑制作用可在任何时候逆转。这些实验表明，不同的GAGs对肌生成有不同的影响，且HA可积极抑制该过程。
