枯草芽孢杆菌孢子形成过程中复制的DNA的密度梯度分析。
Density gradient analysis of DNA replicated during Bacillus subtilis sporulation.
作者信息
Binnie C, Coote J G
出版信息
J Bacteriol. 1983 Oct;156(1):466-70. doi: 10.1128/jb.156.1.466-470.1983.
Abstract
Density gradient centrifugation was used to monitor DNA replication during sporulation of a 5-bromo-2'-deoxyuridine-tolerant, thymidine-requiring strain of Bacillus subtilis. DNA of heavy, intermediate, and light density was found in cells induced to sporulate in the presence of bromodeoxyuridine, but only intermediate DNA was detected in mature spores. Cells grown with bromodeoxyuridine until DNA was in the heavy form formed spores containing intermediate and light DNA when sporulated with thymidine alone.
摘要
利用密度梯度离心法监测枯草芽孢杆菌的一株耐5-溴-2'-脱氧尿苷、需胸腺嘧啶核苷的菌株在芽孢形成过程中的DNA复制。在溴脱氧尿苷存在的情况下诱导形成芽孢的细胞中发现了重密度、中等密度和轻密度的DNA，但在成熟芽孢中仅检测到中等密度的DNA。用溴脱氧尿苷培养至DNA呈重链形式的细胞，在仅用胸腺嘧啶核苷诱导形成芽孢时，会形成含有中等密度和轻密度DNA的芽孢。
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