Binnie C, Coote J G
J Gen Microbiol. 1986 Feb;132(2):493-502. doi: 10.1099/00221287-132-2-493.
A 5-bromo-2'-deoxyuridine (BUdR)-tolerant derivative of a thymidine (TdR)-requiring strain of Bacillus subtilis was used to examine the effect of BUdR, an analogue of TdR, on sporulation. At a TdR:BUdR ratio which had little effect on growth, sporulation was inhibited if cells were exposed to BUdR during the period of DNA synthesis at the onset of the process. Cells recovered from BUdR inhibition of sporulation if the analogue was removed and DNA replication allowed to continue with TdR alone. BUdR prolonged the period of DNA synthesis during sporulation and experiments with chloramphenicol suggested that this was due in part to unscheduled initiation of new rounds of replication.
利用枯草芽孢杆菌胸腺嘧啶核苷（TdR）需求菌株的5-溴-2'-脱氧尿苷（BUdR）耐受衍生物，来研究胸腺嘧啶核苷类似物BUdR对芽孢形成的影响。在对生长影响很小的TdR:BUdR比例下，如果在芽孢形成过程开始时的DNA合成期将细胞暴露于BUdR，芽孢形成会受到抑制。如果去除该类似物并仅用TdR使DNA复制继续进行，细胞可从BUdR对芽孢形成的抑制中恢复。BUdR延长了芽孢形成过程中DNA合成的时间，氯霉素实验表明，这部分是由于新一轮复制的异常起始所致。
