Feilij H, Muller L, Gonzalez Cappa S M
J Clin Microbiol. 1983 Aug;18(2):327-30. doi: 10.1128/jcm.18.2.327-330.1983.
A microhematocrit concentration method (MH) for immediate diagnosis of Chagas' disease during the acute stage or in congenital cases was standardized. Parasitemia as low as 1,000 parasites per ml was detected, after centrifugation of six 50-microliters capillary tubes, by 10-min microscopic observation of each buffy coat spread between slide and cover glass. Operator's time was reduced by at least one-third when compared with a fresh blood observation (FB). In 12 of the 15 patients studied, diagnosis was performed in 4.9 +/- 3.08 min with MH, whereas 27.0 +/- 12.1 min were necessary when FB was used. In the three remaining patients whose FB results were negative, MH became positive after 13, 16, and 40 min. In our experience, FB proved to be more sensitive than previously reported. Suckling mouse inoculation also proved to be sensitive but, as in xenodiagnosis and in hemoculture, the delay in getting the final result was a limiting factor.
一种用于急性期或先天性恰加斯病即时诊断的微量血细胞比容浓缩法（MH）得到了标准化。通过对六支50微升毛细管进行离心，然后对每张载玻片和盖玻片之间涂抹的白细胞层进行10分钟显微镜观察，能够检测到低至每毫升1000个寄生虫的虫血症。与新鲜血液观察法（FB）相比，操作人员的时间至少减少了三分之一。在研究的15名患者中，有12名患者使用MH在4.9 +/- 3.08分钟内完成诊断，而使用FB时则需要27.0 +/- 12.1分钟。其余三名FB结果为阴性的患者，使用MH在13、16和40分钟后呈阳性。根据我们的经验，FB比之前报道的更敏感。乳鼠接种也被证明是敏感的，但与异种诊断和血液培养一样，获得最终结果的延迟是一个限制因素。
