Huser H, Goeke L, Karst G, Fehrenbach F J
J Gen Microbiol. 1983 May;129(5):1295-300. doi: 10.1099/00221287-129-5-1295.
Streptococcus agalactiae (group B) was grown in Todd-Hewitt broth (36.4 g l-1, pH 7.8) in a Braun Fermenter (type B20) to investigate the conditions of optimal bacterial growth and maximal production of CAMP factor. The influence of different gas atmospheres (air, N2, CO2, and gas mixtures) on growth, CAMP production and chain length of S. agalactiae was studied. The organisms grew best in the presence of 2% (w/v) glucose, at pH 6.2, with a constant flow of CO2. The number of diplococci and monococci under these conditions reached almost 80% of the total population.
无乳链球菌（B组）在B型布劳恩发酵罐（B20型）中的托德-休伊特肉汤（36.4 g l-1，pH 7.8）中培养，以研究最佳细菌生长条件和CAMP因子的最大产量。研究了不同气体环境（空气、N2、CO2和气体混合物）对无乳链球菌生长、CAMP产生和链长度的影响。该微生物在2%（w/v）葡萄糖存在下、pH 6.2且有恒定CO2流的条件下生长最佳。在这些条件下，双球菌和单球菌的数量几乎达到了总菌数的80%。
