Labeling and quantitation of ornithine decarboxylase protein by reaction with alpha-[5-14C]difluoromethylornithine.
作者信息
Seely J E, Pösö H, Pegg A E
出版信息
Methods Enzymol. 1983;94:206-9. doi: 10.1016/s0076-6879(83)94034-x.
PMID:6413816
Abstract
摘要
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Labeling and quantitation of ornithine decarboxylase protein by reaction with alpha-[5-14C]difluoromethylornithine.
Methods Enzymol. 1983;94:206-9. doi: 10.1016/s0076-6879(83)94034-x.
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Autoradiographic identification of ornithine decarboxylase in mouse kidney by means of alpha-[5-14C]difluoromethylornithine.通过α-[5-¹⁴C]二氟甲基鸟氨酸对小鼠肾脏中鸟氨酸脱羧酶进行放射自显影鉴定。
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Autoradiographic localization of ornithine decarboxylase in mouse kidney by use of radiolabeled alpha-difluoromethylornithine.利用放射性标记的α-二氟甲基鸟氨酸对小鼠肾脏中鸟氨酸脱羧酶进行放射自显影定位。
Cell Tissue Res. 1984;235(2):371-7. doi: 10.1007/BF00217862.
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Purification of ornithine decarboxylase from kidneys of androgen-treated mice.从雄激素处理过的小鼠肾脏中纯化鸟氨酸脱羧酶。
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Measurement of the number of ornithine decarboxylase molecules in rat and mouse tissues under various physiological conditions by binding of radiolabelled alpha-difluoromethylornithine.通过放射性标记的α-二氟甲基鸟氨酸结合来测定不同生理条件下大鼠和小鼠组织中鸟氨酸脱羧酶分子的数量。
Biochem J. 1982 Aug 15;206(2):311-8. doi: 10.1042/bj2060311.
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Chinese hamster ovary cells resistant to alpha-difluoromethylornithine are overproducers of ornithine decarboxylase.对α-二氟甲基鸟氨酸具有抗性的中国仓鼠卵巢细胞是鸟氨酸脱羧酶的过量生产者。
J Biol Chem. 1983 Oct 25;258(20):12601-8.
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Studies of mammalian ornithine decarboxylase using a monoclonal antibody.使用单克隆抗体对哺乳动物鸟氨酸脱羧酶的研究。
Biochem J. 1984 Jan 1;217(1):123-8. doi: 10.1042/bj2170123.
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Measurement of the amount of ornithine decarboxylase in Saccharomyces cerevisiae and Saccharomyces uvarum by using alpha-[5-14C]difluoromethylornithine.使用α-[5-¹⁴C]二氟甲基鸟氨酸测定酿酒酵母和葡萄汁酵母中鸟氨酸脱羧酶的含量。
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alpha-Difluoromethylornithine does not bind to ornithine decarboxylase-antizyme complex.α-二氟甲基鸟氨酸不与鸟氨酸脱羧酶-抗酶复合物结合。
Biochem Biophys Res Commun. 1986 Jun 30;137(3):1101-5. doi: 10.1016/0006-291x(86)90338-4.
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High level expression in Escherichia coli of soluble, enzymatically active schistosomal hypoxanthine/guanine phosphoribosyltransferase and trypanosomal ornithine decarboxylase.可溶性、具有酶活性的血吸虫次黄嘌呤/鸟嘌呤磷酸核糖基转移酶和锥虫鸟氨酸脱羧酶在大肠杆菌中的高水平表达。
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