Paul J, Kinsella J E
Prostaglandins Leukot Med. 1983 Aug;11(4):419-30. doi: 10.1016/0262-1746(83)90096-3.
The isolated perfused rat heart was used to study if arachidonic acid (AA) release affected prostaglandin (PG) production. The perfused heart avidly absorbed and esterified exogenous AA, mostly into polar lipids. The administration of bradykinin (BK) after prelabeling with 1-C14-AA caused a two-fold increase in the amount of AA released. Bovine serum albumin (BSA) stimulated release 2.5-fold. Perfusion with AA saturated BSA enhanced the release of 1-14C-AA 23-fold indicating the presence of an active deacylation/reacylation system in rat heart. BK caused a four-fold increase whereas BSA had no effect on the amount of prostaglandins released from perfused heart indicating that BK acted on a specific AA pool available to cyclooxygenase whereas the BSA stimulated a separate pool of AA which apparently was not available to the cyclooxygenase.
采用离体灌注大鼠心脏来研究花生四烯酸（AA）释放是否会影响前列腺素（PG）的产生。灌注心脏能大量吸收并酯化外源性AA，主要是将其转化为极性脂质。在用1-C14-AA预标记后给予缓激肽（BK），导致释放的AA量增加了两倍。牛血清白蛋白（BSA）刺激释放增加2.5倍。用AA饱和的BSA灌注可使1-14C-AA的释放增加23倍，这表明大鼠心脏中存在一个活跃的去酰化/再酰化系统。BK使释放量增加了四倍，而BSA对灌注心脏释放的前列腺素量没有影响，这表明BK作用于环氧化酶可利用的特定AA池，而BSA刺激的是一个显然不能被环氧化酶利用的单独AA池。
