Puustinen T, Dahl M L, Uotila P
Prostaglandins. 1983 Jun;25(6):829-37. doi: 10.1016/0090-6980(83)90007-2.
Isolated hamster lungs were labelled with 14C-arachidonic acid. When the lungs were ventilated with a respirator only a small amount of radioactivity was released to the perfusion effluent. This release was not changed significantly by pulmonary infusion of quinacrine (0.5 mM), a known inhibitor of phospholipase A2. After the perfusion about 75% of the radioactivity in the lungs was in phospholipids, mainly in phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol and to a lesser degree in phosphatidylserine and phosphatidic acid. About one fourth of the radioactivity was in neutral lipids (tri- and diacylglycerols) and as free unmetabolized 14C-arachidonic acid. Pulmonary infusion of quinacrine increased the amount of radioactivity in diacylglycerols and phosphatidylinositol but had no effect on that in phosphatidylcholine, phosphatidylserine, phosphatidic acid and triacylglycerols. The amount of radioactivity in phosphatidylethanolamine was decreased by quinacrine and increased in the vicinity of an unidentified phospholipid-quinacrine complex. The present study indicates that the distribution of 14C-arachidonic acid in hamster lung lipids is sensitive to quinacrine. The detected changes can, however, not be explained by an overall inhibition of phospholipase A2 activities.
将离体仓鼠肺用14C-花生四烯酸进行标记。当仅用呼吸机对肺进行通气时，仅有少量放射性物质释放到灌注流出液中。用喹吖因（0.5 mM）进行肺灌注，喹吖因是一种已知的磷脂酶A2抑制剂，这种释放并没有显著改变。灌注后，肺中约75%的放射性物质存在于磷脂中，主要是磷脂酰胆碱、磷脂酰乙醇胺和磷脂酰肌醇，而磷脂酰丝氨酸和磷脂酸中的含量较少。约四分之一的放射性物质存在于中性脂质（甘油三酯和二酰基甘油）以及游离的未代谢14C-花生四烯酸中。肺灌注喹吖因增加了二酰基甘油和磷脂酰肌醇中的放射性物质含量，但对磷脂酰胆碱、磷脂酰丝氨酸、磷脂酸和甘油三酯中的放射性物质含量没有影响。喹吖因使磷脂酰乙醇胺中的放射性物质含量降低，而在一种未鉴定的磷脂 - 喹吖因复合物附近含量增加。本研究表明，14C-花生四烯酸在仓鼠肺脂质中的分布对喹吖因敏感。然而，检测到的变化不能用磷脂酶A2活性的全面抑制来解释。
