Carlebjörk G, Blombäck M, Akerblom O
Vox Sang. 1983;45(3):233-42. doi: 10.1111/j.1423-0410.1983.tb01909.x.
Blood collected into different anticoagulants was stored in small tubes at +4 degrees C for up to 26 h. Seven blood coagulation analyses were performed under standardized conditions. High yield and stability of factor VIII:C were found for ACD and CPD-adenine. No changes could be found in the other six parameters tested. Whole blood in blood bags could be stored for 2-4 h at +4 degrees C with maximal yield of F VIII:C, with blood stored overnight the recovery was 65%. In plasma F VIII:C was stable for at least 2 h at room temperature. F VIIIR:Ag and F VIIIR:RCoF were stable in both whole blood and plasma. No activation by plasmin as measured by B beta 15-42 could be demonstrated. The initial FPA levels, reflecting thrombin activation, in the donated blood differed individually and in some blood bags very high concentrations were found. The levels of FPA were not correlated to the time for collection of a bag of blood.
采集到不同抗凝剂中的血液在小试管中于4℃保存长达26小时。在标准化条件下进行了七次凝血分析。发现对于ACD和CPD -腺嘌呤，因子VIII:C具有高产量和稳定性。在测试的其他六个参数中未发现变化。血袋中的全血在4℃可保存2 - 4小时，此时因子VIII:C产量最高，过夜保存的血液其回收率为65%。在血浆中，因子VIII:C在室温下至少稳定2小时。因子VIII相关抗原（F VIIIR:Ag）和因子VIII相关抗原的瑞斯托霉素辅因子（F VIIIR:RCoF）在全血和血浆中均稳定。未证明有纤溶酶激活，通过Bβ15 - 42测量未显示有激活情况。反映凝血酶激活的初始纤维蛋白肽A（FPA）水平在献血中个体差异较大，在一些血袋中发现浓度非常高。FPA水平与一袋血的采集时间无关。
