Bishop R, Martinez R, Nakamura K D, Weber M J
Biochem Biophys Res Commun. 1983 Sep 15;115(2):536-43. doi: 10.1016/s0006-291x(83)80178-8.
The tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate is mitogenic for normal chicken embryo fibroblasts and also causes these cells to express transiently many properties of cells transformed by Rous sarcoma virus. Since some mitogenic hormones stimulate a tyrosine-specific protein kinase activity, and since the transforming protein of RSV is a tyrosine-specific protein kinase, we have examined whether TPA also stimulates protein phosphorylation on tyrosine. We report here that TPA treatment of normal cells resulted in a very rapid phosphorylation on tyrosine of a protein peak of Mr 40 to 43 kilodaltons. Thus, a similar biochemical activity (tyrosine phosphorylation) is associated with the action of polypeptide mitogenic hormones, Rous sarcoma virus and a tumor promoter. In addition, TPA treatment resulted in rapid changes in phosphorylation of proteins on serine and threonine.
肿瘤促进剂12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯对正常鸡胚成纤维细胞有促有丝分裂作用，还能使这些细胞瞬时表达许多劳斯肉瘤病毒转化细胞的特性。由于一些促有丝分裂激素能刺激酪氨酸特异性蛋白激酶活性，且由于劳斯肉瘤病毒的转化蛋白是一种酪氨酸特异性蛋白激酶，我们研究了佛波酯是否也能刺激酪氨酸上的蛋白磷酸化。我们在此报告，用佛波酯处理正常细胞会导致一个分子量为40至43千道尔顿的蛋白峰在酪氨酸上非常快速地磷酸化。因此，一种类似的生化活性（酪氨酸磷酸化）与多肽促有丝分裂激素、劳斯肉瘤病毒及一种肿瘤促进剂的作用相关。此外，用佛波酯处理导致丝氨酸和苏氨酸上的蛋白质磷酸化快速变化。
