Auffray C, Korman A J, Roux-Dosseto M, Schamboeck A, Strominger J L
Hum Immunol. 1983 Sep;8(1):89-93. doi: 10.1016/0198-8859(83)90086-1.
A cDNA clone corresponding to the HLA-DR alpha chain has been isolated following immunoprecipitation of polysomes with a monoclonal antibody against the denatured DR alpha chain. This cDNA clone has been used to isolate and solve the complete structure of the DR alpha chain gene and a cDNA clone corresponding to the DC alpha chain. The DR alpha and the DC alpha chains bear remarkable homology to each other and have one domain (alpha 2) which is a member of the immunoglobulin superfamily. The DC alpha chain gene has been demonstrated to be the homologue of the murine I-A alpha chain gene, and it has been localized on chromosome 6 using deletion mutants. A restriction enzyme polymorphism of the DC alpha chain gene has been detected in HLA-DR homozygous typing cells. This report is a summary of a presentation made at the HLA-DR meeting held in Marseille, France.
利用抗变性DRα链的单克隆抗体对多核糖体进行免疫沉淀后，分离出了一个与HLA - DRα链相对应的cDNA克隆。该cDNA克隆已被用于分离和解析DRα链基因以及与DCα链相对应的cDNA克隆的完整结构。DRα链和DCα链彼此具有显著的同源性，并且有一个结构域（α2）属于免疫球蛋白超家族。DCα链基因已被证明是小鼠I - Aα链基因的同源物，并且利用缺失突变体将其定位在6号染色体上。在HLA - DR纯合分型细胞中检测到了DCα链基因的限制性内切酶多态性。本报告是在法国马赛举行的HLA - DR会议上所做报告的总结。
