Marceau F, Drumheller A, Gendreau M, Lussier A, St-Pierre S
J Chromatogr. 1983 Aug 26;266:173-7. doi: 10.1016/s0021-9673(01)90890-8.
A rapid and sensitive method for measuring carboxypeptidase N (CPN) activity in human plasma is described. The procedure is based on the hydrolysis of a high-specificity/low-affinity substrate, hippuryl-L-lysine, to its products hippuric acid and lysine. The substrate and product are separated quantitatively by high-performance liquid chromatography in less than 10 min following a minimal sample preparation time. The advantages of this method over previous ones are discussed and data are presented demonstrating the reliability of this method for the routine clinical determination of CPN activity.
本文描述了一种快速、灵敏的测定人血浆中羧肽酶N（CPN）活性的方法。该方法基于高特异性/低亲和力底物马尿酸-L-赖氨酸水解为其产物马尿酸和赖氨酸。在最短的样品制备时间后，通过高效液相色谱法在不到10分钟的时间内对底物和产物进行定量分离。讨论了该方法相对于以前方法的优点，并给出了数据，证明了该方法用于CPN活性常规临床测定的可靠性。
