Maseda C, Fukui Y, Kimura K, Matsubara K
J Forensic Sci. 1983 Oct;28(4):911-21.
A simple and sensitive assay for the cannabinoids is presented using a dabsylation procedure. Dabsyl derivatives of delta 9-tetrahydrocannabinol (delta 9-THC) and cannabinol (CBN) were prepared by reacting with 4-dimethylaminoazobenzene-4'-sulfonyl chloride (dabsyl chloride) in acetone in the presence of sodium carbonate-sodium bicarbonate buffer (pH 10). Crystalline dabsylcannabinoids gave intense absorption in the visible region. With these derivatives, analysis by thin-layer chromatography (TLC) and high performance liquid chromatography (HPLC) were tested. These techniques gave good separation and nanogram detection of dabsyl-THC and -CBN by using n-hexane-ethyl acetate-diethylamine (20:5:1) for TLC and MeOH--H2O (95:5) at 450 nm for HPLC.
本文介绍了一种使用丹磺酰化程序对大麻素进行简单且灵敏的检测方法。通过在丙酮中，于碳酸钠 - 碳酸氢钠缓冲液（pH 10）存在的条件下，使δ9 - 四氢大麻酚（δ9 - THC）和大麻酚（CBN）与4 - 二甲基氨基偶氮苯 - 4'- 磺酰氯（丹磺酰氯）反应，制备出δ9 - THC和CBN的丹磺酰衍生物。结晶状的丹磺酰化大麻素在可见光区域有强烈吸收。利用这些衍生物，对薄层色谱法（TLC）和高效液相色谱法（HPLC）的分析进行了测试。通过使用正己烷 - 乙酸乙酯 - 二乙胺（20:5:1）进行TLC分析，以及在450 nm波长下使用甲醇 - 水（95:5）进行HPLC分析，这些技术对丹磺酰 - THC和 - CBN实现了良好的分离以及纳克级别的检测。
