Chvapil M, Speer D, Mora W, Eskelson C
J Surg Res. 1983 Nov;35(5):402-9. doi: 10.1016/0022-4804(83)90029-x.
Pure collagen, isolated from bovine skin, was reconstituted into the form of a sponge in the presence of either glutaraldehyde (GTA) or hexamethylene diisocyanate (DIC). Extensively washed sponges were implanted subcutaneously in rats and harvested 5 and 17 days later. Histology showed that at 5 days, the GTA-crosslinked sponge induced more cellular reaction at the outer layer of the sponge than the DIC-tanned sponge. After 17 days, the cellular infiltration of the GTA-tanned sponge remained at the periphery of the implant while the DIC-tanned sponge was completely infiltrated by inflammatory cells, including fibroblasts. Quantitative morphometry and determination of cellular DNA in sponges harvested at 17 days support the morphological finding. We conclude that GTA-tanned sponges are cytotoxic as evidenced by more pronounced tissue reaction soon after tissue implantation, and no cellular infiltration at later stages into the implant. Hexamethylene diisocyanate seems to be a more adequate tanning agent for sponges designed as a tissue substitute.
从牛皮中分离出的纯胶原蛋白，在戊二醛（GTA）或六亚甲基二异氰酸酯（DIC）存在的情况下被重构为海绵形式。经过充分洗涤的海绵被皮下植入大鼠体内，并在5天和17天后取出。组织学显示，在5天时，GTA交联的海绵在海绵外层诱导的细胞反应比DIC鞣制的海绵更多。17天后，GTA鞣制的海绵的细胞浸润仍留在植入物的周边，而DIC鞣制的海绵则被包括成纤维细胞在内的炎性细胞完全浸润。对17天取出的海绵进行的定量形态测量和细胞DNA测定支持了形态学发现。我们得出结论，GTA鞣制的海绵具有细胞毒性，这在组织植入后不久更明显的组织反应以及后期植入物中无细胞浸润中得到证明。对于设计用作组织替代物的海绵，六亚甲基二异氰酸酯似乎是一种更合适的鞣制剂。
