Slobodyanyuk S Y, Serov O L
Mol Gen Genet. 1983;191(3):372-7. doi: 10.1007/BF00425748.
The effects of chromosomal rearrangements on the expression of the gene Pgd coding for 6-phosphogluconate dehydrogenase (PGD) was studied in D. melanogaster. Of 21 chromosomal rearrangements examined, 2 produced complete loss of PGD activity, 10 markedly decreased it, 3 slightly increased it, and 6 had no appreciable effect. The effect of some rearrangements can be restricted to the larval stage. The results of histochemical staining and the quantitative analysis of the electrophoretic PGD patterns allowed us to identify chromosomal rearrangements whose inhibition of PGD activity was due to mosaic expression of the Pgd gene. The rearrangements whose effects are variegated represented 50% of those decreasing the expression of the marker gene or about 25% of those we considered.
在黑腹果蝇中研究了染色体重排对编码6-磷酸葡萄糖酸脱氢酶（PGD）的Pgd基因表达的影响。在所检测的21种染色体重排中，2种导致PGD活性完全丧失，10种使其显著降低，3种使其略有增加，6种没有明显影响。一些重排的影响可能仅限于幼虫阶段。组织化学染色结果和PGD电泳图谱的定量分析使我们能够鉴定出其对PGD活性的抑制是由于Pgd基因的镶嵌表达所致的染色体重排。其影响呈斑驳状的重排占降低标记基因表达的重排的50%，或占我们所研究的重排的约25%。
