葡萄糖庚糖酸盐与锝-99m的电解络合

Electrolytic complexing of glucoheptonate and technetium-99m.

作者信息

Chi S L, Hoag S G, Yanchick V A

出版信息

J Nucl Med. 1978 May;19(5):520-4.

Abstract

Solutions of glucoheptonate and sodium pertechnetate (Tc-99m) were subjected to electrolysis at various ampere-time products until a charge was found that consistently promoted tagging of greater than 90% efficiency. It was found that 9 coulombs (100 mA, 90 sec) consistently yielded a final product that contained less than 10% total radiochemical impurities (unbound pertechnetate and reduced, hydrolyzed technetium). Radiochemical purity of the final product was established using a two-solvent thin-layer chromatographic system with methyl-ethyl ketone and normal saline as the solvents. The tagging efficiency and stability of the tagged complex were determined with similar chromatographic analysis. It was shown that use of a 15% solution of calcium glucoheptonate resulted in a more stable product than that prepared from commercially available stannous glucoheptonate. The rapid, accurate chromatographic method for determination or radiochemical purity of the product is described. The final product is considered equal or superior to commercial Tc-99m (Sn) glucoheptonate and was produced at considerably less cost.

摘要

将葡萄糖庚糖酸盐和高锝酸钠（Tc-99m）溶液在不同的安培-时间乘积下进行电解，直到发现一种能持续促进标记效率大于90%的电荷量。结果发现，9库仑（100 mA，90秒）能持续产生一种最终产物，其总放射化学杂质（未结合的高锝酸盐以及还原、水解的锝）含量低于10%。使用以甲基乙基酮和生理盐水为溶剂的双溶剂薄层色谱系统来确定最终产物的放射化学纯度。用类似的色谱分析来测定标记复合物的标记效率和稳定性。结果表明，使用15%的葡萄糖庚糖酸钙溶液得到的产物比用市售葡萄糖庚糖酸亚锡制备的产物更稳定。本文描述了用于测定产物放射化学纯度的快速、准确的色谱方法。最终产物被认为等同于或优于市售的Tc-99m（Sn）葡萄糖庚糖酸盐，并且生产成本要低得多。