怀孕和未怀孕兔子子宫的前列腺素生成

Prostaglandin production by the pregnant and non-pregnant rabbit uterus.

作者信息

Bloch M H, McLaughlin L L, Martin S A, Needleman P

出版信息

Prostaglandins. 1983 Jul;26(1):33-46. doi: 10.1016/0090-6980(83)90072-2.

Abstract

We have studied the prostaglandin synthesis of the pregnant and non-pregnant rabbit uterus in a microsomal membrane preparation, and in an ex vivo perfused uterus preparation which retains agonist stimulated prostaglandin production. In both the microsomal and isolated perfused system, prostacyclin was the major arachidonic acid metabolite produced; PGE2 was also produced in substantial quantities while TxB2 and PGF2 alpha were not detectable. Moreover, oxytocin was a specific stimulus of PGE2 release. The steroid hormone milieu influenced the level of agonist stimulated prostaglandin release; in general, ovariectomized, estrogen treated animals were more responsive to agonist stimulation than those treated with estrogen followed by progesterone. The microsomal studies indicated that the pregnant animal had a greatly enhanced capacity to metabolize arachidonic acid when compared with the non-pregnant animal. However, this was not reflected in the ability of agonists to stimulate prostaglandin release in the ex vivo perfused preparation.

摘要

我们在微粒体膜制剂以及保留激动剂刺激前列腺素生成的离体灌注子宫制剂中，研究了妊娠和未妊娠兔子宫的前列腺素合成。在微粒体和离体灌注系统中，前列环素是产生的主要花生四烯酸代谢产物；也大量产生了PGE2，而未检测到TxB2和PGF2α。此外，催产素是PGE2释放的特异性刺激物。类固醇激素环境影响激动剂刺激的前列腺素释放水平；一般而言，去卵巢并用雌激素处理的动物比先用雌激素再用孕酮处理的动物对激动剂刺激更敏感。微粒体研究表明，与未妊娠动物相比，妊娠动物代谢花生四烯酸的能力大大增强。然而，这并未反映在激动剂刺激离体灌注制剂中前列腺素释放的能力上。