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单细胞四膜虫中的凝集素。I. 用异硫氰酸荧光素标记的抗凝集素检测凝集素

Lectins in the unicellular Tetrahymena. I. Lectin detection with FITC-labeled anti-lectins.

作者信息

Csaba G, Kovács P

出版信息

Acta Histochem. 1983;73(1):53-61. doi: 10.1016/S0065-1281(83)80075-0.

Abstract

FITC-labeled antibodies prepared in rabbits to bean, pea, lens, datura, and snail lectin indicated by immunological reaction presence in the Tetrahymena of corresponding lectin-like components. The latter localized partly on the surface, partly inside the body of the unicellular. The specificity of the anti-lectins was ascertained by immunological and hemagglutination tests, and by examining antibody binding after pretreatment of the Tetrahymena with lectin or sugar.

摘要

用兔制备的针对豆、豌豆、晶状体、曼陀罗和蜗牛凝集素的异硫氰酸荧光素标记抗体,通过免疫反应表明在四膜虫中存在相应的凝集素样成分。后者部分定位于单细胞的表面,部分定位于其体内。通过免疫和血凝试验,以及在用凝集素或糖预处理四膜虫后检测抗体结合,确定了抗凝集素的特异性。

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