Shoupe D, Montz F J, Kletzky O A, diZerega G S
Am J Obstet Gynecol. 1983 Nov 1;147(5):482-7.
Since the milieu of pregnancy stimulates physiologic hyperprolactinemia, we questioned whether prolactin secreted during normal pregnancy contains a large-molecular weight component that binds to concanavalin A and whether this large-molecular weight prolactin contributes to the thyrotropin-releasing hormone (TRH)-releasable pool. Serum was collected from pregnant patients (n = 28) undergoing TRH stimulation tests. This serum was passed through a concanavalin A column and eluted with 0.2M alpha-methylmannoside. Concanavalin A-bound prolactin, as determined by radioimmunoassay, ranged from 10% to 30% of the total immunoassayable prolactin. An increase in the basal serum concentration of both concanavalin A-bound and -unbound prolactin occurred as pregnancy progressed. However, throughout gestation, only the concanavalin A-unbound prolactin increased after TRH stimulation. The concanavalin A-bound prolactin was found to have a molecular weight of 60,000 by means of Sephadex G-100 permeation chromatography.
由于孕期环境会刺激生理性高催乳素血症，我们不禁要问，正常孕期分泌的催乳素是否包含与伴刀豆球蛋白A结合的大分子成分，以及这种大分子催乳素是否对促甲状腺激素释放激素（TRH）可释放池有贡献。从接受TRH刺激试验的孕妇（n = 28）中采集血清。该血清通过伴刀豆球蛋白A柱，并以0.2Mα-甲基甘露糖苷洗脱。通过放射免疫测定法测定，与伴刀豆球蛋白A结合的催乳素占总免疫可测定催乳素的10%至30%。随着孕期进展，与伴刀豆球蛋白A结合和未结合的催乳素的基础血清浓度均升高。然而，在整个妊娠期，TRH刺激后只有未与伴刀豆球蛋白A结合的催乳素增加。通过Sephadex G - 100渗透色谱法发现，与伴刀豆球蛋白A结合的催乳素分子量为60,000。
