Duvall E J, Williams D M, Lovett P S, Rudolph C, Vasantha N, Guyer M
Gene. 1983 Oct;24(2-3):171-7. doi: 10.1016/0378-1119(83)90077-x.
A cloned Bacillus pumilus cat gene expresses chloramphenicol-inducible chloramphenicol acetyltransferase activity in Bacillus subtilis. The chloramphenicol inducibility trait was shown to be determined by a 234-bp region of the cloned DNA. Nucleotide sequence analysis of this 234-bp segment indicated that the cat ribosome-binding site occurs within a 40-bp region containing 14-bp terminal inverted repeat sequences. Transcription of this region into RNA should sequester the cat ribosome-binding site in a stable stem-loop conformation. Chloramphenicol-mediated destabilization of the stem-loop is suggested as the basis for the chloramphenicol inducibility phenotype.
一个克隆的短小芽孢杆菌cat基因在枯草芽孢杆菌中表达氯霉素诱导型氯霉素乙酰转移酶活性。氯霉素诱导特性被证明是由克隆DNA的一个234 bp区域决定的。对这个234 bp片段的核苷酸序列分析表明，cat核糖体结合位点位于一个40 bp区域内，该区域包含14 bp的末端反向重复序列。该区域转录成RNA后，应会将cat核糖体结合位点隔离在一个稳定的茎环结构中。氯霉素介导的茎环结构不稳定被认为是氯霉素诱导型表型的基础。
