蛋黄培养基用于肺炎支原体培养的效用
Utility of egg yolk medium for cultivation of Mycoplasma pneumoniae.
作者信息
Sasaki T, Shintani M, Kihara K
出版信息
J Clin Microbiol. 1983 Nov;18(5):1167-73. doi: 10.1128/jcm.18.5.1167-1173.1983.
Chicken egg yolk broth medium containing 10% (vol/vol) phosphate-buffered saline extract of egg yolk instead of horse serum showed better growth-promoting activity for Mycoplasma pneumoniae than did Chanock medium and was effective in promoting the growth of small numbers of M. pneumoniae. Moreover, the phosphate-buffered saline extract of egg yolk proved superior to horse serum in the following respects: (i) it was consistent in growth-promoting activity for M. pneumoniae among different batches; (ii) it could be preserved at 4 degrees C for at least 2 years; (iii) it is inexpensive and easy to obtain; and (iv) it contains large amounts of lipoproteins, cholesterol, and phospholipids.
含有10%（体积/体积）蛋黄磷酸盐缓冲盐水提取物而非马血清的鸡蛋黄肉汤培养基对肺炎支原体的促生长活性比查诺克培养基更好，并且在促进少量肺炎支原体生长方面有效。此外，蛋黄磷酸盐缓冲盐水提取物在以下方面被证明优于马血清：（i）在不同批次间对肺炎支原体的促生长活性一致；（ii）可在4℃保存至少2年；（iii）价格低廉且易于获得；（iv）含有大量脂蛋白、胆固醇和磷脂。
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