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雪貂催乳素水平的研究。

Study of prolactin levels in the ferret.

作者信息

Smith S V, Forsyth I A, Donovan B T

出版信息

J Endocrinol. 1983 Dec;99(3):415-21. doi: 10.1677/joe.0.0990415.

DOI:10.1677/joe.0.0990415
PMID:6417258
Abstract

A radioimmunoassay for canine prolactin has been used to measure prolactin in the ferret. Serial dilutions of extracts of ferret pituitary glands and of ferret plasma yielded curves that were parallel with the canine prolactin standard curve. The sensitivity, accuracy, reproducibility and precision of the assay were within acceptable limits. Plasma prolactin levels increased after the administration of thyrotrophin releasing hormone (TRH) or chlorpromazine, but not after giving luteinizing hormone releasing hormone. Female ferrets, which were anoestrous, oestrous or spayed, and male ferrets had similar basal prolactin levels when sampled under sodium pentobarbitone anaesthesia. These basal levels were higher than in conscious males and the latter also showed a lesser response to TRH. Hypophysectomy significantly reduced basal prolactin levels in female ferrets by 2 h postoperatively and abolished the response to TRH.

摘要

一种用于犬催乳素的放射免疫分析法已被用于测量雪貂体内的催乳素。雪貂垂体提取物和雪貂血浆的系列稀释液产生的曲线与犬催乳素标准曲线平行。该分析方法的灵敏度、准确性、可重复性和精密度均在可接受范围内。注射促甲状腺激素释放激素(TRH)或氯丙嗪后,血浆催乳素水平升高,但注射促黄体生成素释放激素后则没有升高。处于乏情期、发情期或已绝育的雌性雪貂以及雄性雪貂在戊巴比妥钠麻醉下采样时,基础催乳素水平相似。这些基础水平高于清醒雄性雪貂,且后者对TRH的反应也较小。垂体切除术在术后2小时显著降低了雌性雪貂的基础催乳素水平,并消除了对TRH的反应。

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1
Study of prolactin levels in the ferret.雪貂催乳素水平的研究。
J Endocrinol. 1983 Dec;99(3):415-21. doi: 10.1677/joe.0.0990415.
2
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