Boyd R L, Ward H A, Muller H K
J Reticuloendothel Soc. 1983 Nov;34(5):383-93.
The role of chicken bursal and thymic reticular epithelial cells (REp cells) in the induction of B- and T-lymphocyte differentiation has been investigated using in vitro culture of these cells. Splenic adherent cells were used as controls. Precursor populations were obtained predominantly from embryonic bone marrow or spleen and incubated with REp cell monolayers for 72 hr. Differentiation was assessed by the expression of specific membrane antigens: chicken B lymphocyte-specific antigen (CBLA) and chicken T lymphocyte-specific antigen (CTLA). Bursal REp cells specifically induced approximately 20-30% of the embryonic cells to express CBLA but had no influence on CTLA expression. Conversely, CTLA, but not CBLA, was induced in approximately 20% of the embryonic cells by the thymic REp cell cultures. No effect on the expression of either antigen was observed with the splenic adherent cells. The induced cells correspond to the first stage of B- and T-lymphocyte differentiation found in vivo. The assay system also facilitated the study of shifts in precursor content of various organs with development. Whereas embryonic bone marrow of all ages studied contained both B- and T-lymphocyte precursors, these were not demonstrable in donors after hatching. Use of 12-day embryonic bursa and thymus suspensions as sources of stem cells indicated that precommitment to the B- or T-lymphocyte lineage is irreversible.
利用鸡法氏囊和胸腺的网状上皮细胞（REp细胞）的体外培养，研究了这些细胞在诱导B淋巴细胞和T淋巴细胞分化中的作用。脾贴壁细胞用作对照。前体细胞群主要取自胚胎骨髓或脾脏，并与REp细胞单层培养72小时。通过特异性膜抗原的表达来评估分化情况：鸡B淋巴细胞特异性抗原（CBLA）和鸡T淋巴细胞特异性抗原（CTLA）。法氏囊REp细胞特异性诱导约20%-30%的胚胎细胞表达CBLA，但对CTLA表达没有影响。相反，胸腺REp细胞培养物可诱导约20%的胚胎细胞表达CTLA，而不诱导CBLA表达。脾贴壁细胞对这两种抗原的表达均无影响。诱导的细胞对应于体内发现的B淋巴细胞和T淋巴细胞分化的第一阶段。该检测系统也有助于研究不同器官前体细胞含量随发育的变化。虽然所有研究年龄的胚胎骨髓都含有B淋巴细胞和T淋巴细胞前体，但在孵化后的供体中无法检测到这些前体。使用12日龄胚胎的法氏囊和胸腺悬液作为干细胞来源表明，对B淋巴细胞或T淋巴细胞谱系的预先决定是不可逆的。
