Goehl T J, Mathur L K, Strum J D, Jaffe J M, Pitlick W H, Shah V P, Poust R I, Colaizzi J L
J Pharm Sci. 1978 Mar;67(3):404-6. doi: 10.1002/jps.2600670336.
A simple and rapid high-pressure liquid chromatographic method was developed for the determination of sulfadiazine, sulfamerazine, and sulfamethazine in human serum. After the trichloroacetic acid precipitation of the serum proteins, an aliquot of the supernate is injected into a high-pressure liquid chromatograph equipped with a reversed-phase microparticulate column and a fixed wavelength UV detector. For each of the three components of trisulfapyrimidines, a linear calibration curve was observed in the 1-30-microgram/ml range, with the precision of the assay estimated to be +/- 2% (RSD). Preliminary pharmacokinetic data are also presented.
建立了一种简单快速的高压液相色谱法，用于测定人血清中的磺胺嘧啶、磺胺甲嘧啶和磺胺二甲嘧啶。血清蛋白经三氯乙酸沉淀后，取一份上清液注入配备反相微粒柱和固定波长紫外检测器的高压液相色谱仪中。对于三种三磺胺嘧啶成分中的每一种，在1-30微克/毫升范围内观察到线性校准曲线，测定的精密度估计为±2%（相对标准偏差）。还给出了初步的药代动力学数据。
