Rossetti Z L, Mercuro G, Rivano C A
Life Sci. 1983 Dec 12;33(24):2387-97. doi: 10.1016/0024-3205(83)90632-x.
A general method for the determination of norepinephrine, epinephrine, dopamine, 3,4-dihydroxyphenylalanine and 3,4-dihydroxyphenylacetic acid is described. It employs the generation of a flow gradient during the analysis by ion-pair reversed phase liquid chromatography. The method allows a reduction of the analysis time maintaining high resolution of the peaks close to the solvent front. The effect of the flow gradient on column efficiency, detector response and retention are evaluated. The application to specimens of amniotic fluid, urine, human plasma and rat brain tissue is shown.
描述了一种测定去甲肾上腺素、肾上腺素、多巴胺、3,4-二羟基苯丙氨酸和3,4-二羟基苯乙酸的通用方法。该方法在离子对反相液相色谱分析过程中采用流动梯度生成技术。该方法能够在保持靠近溶剂前沿的峰具有高分辨率的同时减少分析时间。评估了流动梯度对柱效、检测器响应和保留时间的影响。展示了该方法在羊水、尿液、人血浆和大鼠脑组织样本中的应用。
