采用带电化学检测器的高压液相色谱法测定脑中的甲硫氨酸脑啡肽、去甲肾上腺素、多巴胺、3,4-二羟基苯乙酸（DOPAC）和3-甲氧基-4-羟基苯乙酸（HVA）。
Determination of methionine-enkephalin, norepinephrine, dopamine, 3,4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in brain by high-pressure liquid chromatography with electrochemical detector.
作者信息
Nabeshima T, Hiramatsu M, Noma S, Ukai M, Amano M, Kameyama T
出版信息
Res Commun Chem Pathol Pharmacol. 1982 Mar;35(3):421-42.
High-pressure liquid chromatography with electrochemical detection is utilized in a procedure for the determination of methionine-enkephalin (MET-ENK), norepinephrine (NE), dopamine (DA), 3, 4-dihydroxyphenylacetic acid (DOPAC) and 3-methoxy-4-hydroxyphenylacetic acid (HVA) in same brain tissue samples. The high degree of selectivity and sensitivity (picomole limits for each component) makes this technique directly applicable to extremely small samples. After optimal conditions had been established, the procedure was employed in the determination of MET-ENK, NE, DA, DOPAC and HVA contents in whole brains, 7 discrete brain areas of mice and rats.
采用带电化学检测的高压液相色谱法，对同一脑组织样本中的甲硫氨酸脑啡肽（MET-ENK）、去甲肾上腺素（NE）、多巴胺（DA）、3,4-二羟基苯乙酸（DOPAC）和3-甲氧基-4-羟基苯乙酸（HVA）进行测定。该技术具有高度的选择性和灵敏度（每种成分的检测限为皮摩尔），可直接用于极少量样本。在确定最佳条件后，该方法被用于测定小鼠和大鼠全脑、7个离散脑区中的MET-ENK、NE、DA、DOPAC和HVA含量。
Zotero 插件