Failure of gel filtration and HPLC purification to increase levels of immunoreactive TRH in rat brain extracts.
作者信息
Court J A, Smith A I, McDermott J R, Biggins J A, Edwardson J A
出版信息
Peptides. 1983 Jul-Aug;4(4):585-7. doi: 10.1016/0196-9781(83)90066-9.
PMID:6417633
Abstract
It has recently been reported [4] that purification of acidified methanol extracts of rat cerebellum by gel filtration and high performance liquid chromatography (HPLC) caused a fifty-fold increase in immunoreactive thyrotrophin-releasing hormone (ir-TRH). However either using Sephadex LH20 or SepPak separation prior to HPLC we have failed to find a significant increase in ir-TRH in rat cerebellar or hypothalamic extracts. We conclude that it is valid to measure TRH by radioimmunoassay in unpurified methanolic extracts of brain tissue.
摘要
Zotero 插件