Quantitative disk method for storage of Neisseria gonorrhoeae.

A medium has been devised for the storage of Neisseria gonorrhoeae in the form of quantitated disks at -70 C. The medium consists of bovine serum albumin, 5%; inositol, 10%; glutamate, 5%; gelatin, 10%; and charcoal, 0.3%. Thirty-one strains of N. gonorrhoeae have been stored for 12 months and seven strains for 20 months. The organisms have maintained good viability and stability of colonial morphology. This procedure provides a means of maintaining high-density populations of freshly isolated strains of N. gonorrhoeae in quantitated form without subculture.