Butterfield A G, Lovering E G, Sears R W
J Pharm Sci. 1978 May;67(5):650-3. doi: 10.1002/jps.2600670520.
A high-performance liquid chromatographic procedure is presented for the simultaneous determination of reserpine and hydrochlorothiazide in two-component tablet formulations. An aliquot of a tetrahydrofuran extract of the tablet, containing polylythiazide as an internal standard, is chromatographed on a microparticulate silica gel column using a mobile phase of 0.01% (v/v) diethylamine, 5% (v/v) chloroform, and 18% (v/v) 2-propanol in n-hexane. The relative standard deviations are 1.2 and 0.6% for the simultaneous determination of reserpine and hydrochlorothiazide, respectively. Seven commericial tablet formulations were found to contain 92.7--101.0% and 98.3--101.4% of the labeled amounts of reserpine and hydrochlorothiazide, respectively.
介绍了一种高效液相色谱法，用于同时测定两组分片剂制剂中的利血平和氢氯噻嗪。取含有聚硫噻嗪作为内标的片剂四氢呋喃提取物的等分试样，在微粒硅胶柱上进行色谱分析，流动相为0.01%（v/v）二乙胺、5%（v/v）氯仿和18%（v/v）异丙醇的正己烷溶液。利血平和氢氯噻嗪同时测定的相对标准偏差分别为1.2%和0.6%。发现七种市售片剂制剂中利血平和氢氯噻嗪的含量分别为标示量的92.7%-101.0%和98.3%-101.4%。
