Feierman D E, Cederbaum A I
Biochem Biophys Res Commun. 1983 Oct 31;116(2):765-70. doi: 10.1016/0006-291x(83)90590-9.
Rat liver microsomes catalyzed an NADPH-dependent oxidation of dimethylsulfoxide, 2-keto-4-thiomethylbutyrate and ethanol. The addition of EDTA and iron (ferric)-EDTA increased the oxidation of the hydroxyl radical scavenging agents and ethanol. Unchelated iron had no effect; therefore, appropriately chelated iron is required to stimulate microsomal production of hydroxyl radicals. Catalase strongly inhibited control rates as well as EDTA or iron-EDTA stimulated rates of hydroxyl radical production whereas superoxide dismutase had no effect. The rate of ethanol oxidation was ten- to twenty-fold greater than the rate of oxidation of hydroxyl radical scavengers in the absence of EDTA or iron-EDTA, suggesting little contribution by hydroxyl radicals in the pathway of ethanol oxidation. In the presence of EDTA or iron-EDTA, the rate of ethanol oxidation increased, and under these conditions, hydroxyl radicals appear to play a more significant role in contributing toward the overall oxidation of ethanol.
大鼠肝脏微粒体催化了依赖于NADPH的二甲基亚砜、2-酮-4-硫代甲基丁酸酯和乙醇的氧化反应。添加乙二胺四乙酸（EDTA）和铁（三价铁）-EDTA可增加羟基自由基清除剂和乙醇的氧化反应。未螯合的铁没有作用；因此，需要适当螯合的铁来刺激微粒体产生羟基自由基。过氧化氢酶强烈抑制对照速率以及EDTA或铁-EDTA刺激的羟基自由基产生速率，而超氧化物歧化酶没有作用。在不存在EDTA或铁-EDTA的情况下，乙醇氧化速率比羟基自由基清除剂的氧化速率高10至20倍，这表明在乙醇氧化途径中羟基自由基的贡献很小。在存在EDTA或铁-EDTA的情况下，乙醇氧化速率增加，在这些条件下，羟基自由基似乎在乙醇的整体氧化中发挥更重要的作用。
