Cech P, Lehrer R I
Blood. 1984 Jan;63(1):88-95.
Human neutrophils sequester yeast cells or zymosan particles in two classes of phagocytic vacuoles: sealed and unsealed. The pH of sealed vacuoles was measured by a newly devised fluorometric procedure that used fluoresceinated zymosan particles, derived from Saccharomyces cerevisiae, as a pH probe. Five minutes after initiation of phagocytosis, sealed vacuoles were alkaline, exhibiting a pH of 7.80 +/- 0.19 (mean +/- SEM). Their acidity subsequently increased, so that the pH fell to 7.38 +/- 0.25 after 15 min. 6.35 +/- 0.35 after 30 min, and 5.68 +/- 0.26 after 60 min. The implications of these findings for neutrophil microbicidal mechanisms are discussed.
封闭的和未封闭的。通过一种新设计的荧光测定法测量封闭吞噬泡的pH值，该方法使用源自酿酒酵母的荧光标记酵母聚糖颗粒作为pH探针。吞噬作用开始5分钟后，封闭吞噬泡呈碱性，pH值为7.80±0.19（平均值±标准误）。随后其酸度增加，因此15分钟后pH值降至7.38±0.25，30分钟后为6.35±0.35，60分钟后为5.68±0.26。讨论了这些发现对中性粒细胞杀菌机制的影响。
