Barraquio W L, Ladha J K, Watanabe I
Can J Microbiol. 1983 Aug;29(8):867-73. doi: 10.1139/m83-141.
Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.
用添加了葡萄糖的半固体酵母提取物培养基和胰蛋白胨大豆琼脂，从湿地水稻根中需氧分离固氮（乙炔还原）异养细菌。通过凝胶免疫扩散和荧光抗体技术，并结合其形态、培养和生化特征，将分离菌株鉴定为假单胞菌属。本文所述的利用氢气的固氮假单胞菌是一个新物种。
