Sugita T, Stenmark K R, Wagner W W, Henson P M, Henson J E, Hyers T M, Reeves J T
Exp Lung Res. 1983 Nov;5(3):201-15. doi: 10.3109/01902148309061515.
Monocrotaline given to rats causes lung injury which is followed by pulmonary hypertension. A large, abnormal intraalveolar cell has been repeatedly observed. The purpose of the present study was to define the nature of the cell and to determine how it related to the lung injury. As observed by electron microscopy, the intraalveolar cell contained numerous large, dense granules, as well as lipid whorls, extensive Golgi complexes, and many small vesicles. These cells appeared to be macrophages, possibly altered by ingestion of lipid-like material which was free in the alveolar space. Enlarged type II pneumonocytes and mast cells were seen in the alveolar walls but their appearance differed from that of the free alveolar cells. The electron microscopic findings plus the presence of Fc and C3b receptors on the cells indicated they were alveolar macrophages. When the severity of pulmonary hypertension was varied by a) giving monocrotaline to rats of varying age, b) by varying the dose of monocrotaline, or c) by varying the time interval after monocrotaline administration, the number of abnormal alveolar macrophages related to the severity of the pulmonary hypertension. When we reduced the number of circulating leukocytes by whole body radiation, monocrotaline administration was followed both by accelerated pulmonary hypertension and increased numbers of abnormal alveolar macrophages. The abnormal macrophage appeared to be a marker of the monocrotaline induced pulmonary hypertension. However, neither the abnormal macrophage nor the monocrotaline injury appeared to depend on circulating leukocytes.
给大鼠注射野百合碱会导致肺损伤，继而引发肺动脉高压。人们反复观察到一种大型的、异常的肺泡内细胞。本研究的目的是确定该细胞的性质，并确定其与肺损伤的关系。通过电子显微镜观察，肺泡内细胞含有大量大的、致密的颗粒，以及脂滴、广泛的高尔基体复合体和许多小泡。这些细胞似乎是巨噬细胞，可能因摄取肺泡腔内游离的类脂物质而发生了改变。在肺泡壁上可见肿大的II型肺上皮细胞和肥大细胞，但它们的外观与游离的肺泡内细胞不同。电子显微镜检查结果加上细胞表面存在Fc和C3b受体表明它们是肺泡巨噬细胞。当通过以下方式改变肺动脉高压的严重程度时：a）给不同年龄的大鼠注射野百合碱，b）改变野百合碱的剂量，或c）改变注射野百合碱后的时间间隔，异常肺泡巨噬细胞的数量与肺动脉高压的严重程度相关。当我们通过全身辐射减少循环白细胞的数量时，注射野百合碱后会出现肺动脉高压加速和异常肺泡巨噬细胞数量增加的情况。异常巨噬细胞似乎是野百合碱诱导的肺动脉高压的一个标志物。然而，异常巨噬细胞和野百合碱损伤似乎都不依赖于循环白细胞。
