Malkov S V, Prozorov A A
Genetika. 1983 Nov;19(11):1753-9.
In this work, we tried to clone some Bacillus mesentericus genes coding for tryptophan synthesis in Bacillus subtilis cells. We succeeded in obtaining two identical plasmids carrying some genes of Bac. mesentericus and complementing the trpC2 and trpF mutations of Bac. subtilis. The cloned genes of Bac. mesentericus completely replaced the functions of trpC2 and trpF genes.
在这项工作中，我们试图在枯草芽孢杆菌细胞中克隆一些编码色氨酸合成的肠系膜芽孢杆菌基因。我们成功获得了两个携带肠系膜芽孢杆菌某些基因的相同质粒，这些质粒能够互补枯草芽孢杆菌的trpC2和trpF突变。肠系膜芽孢杆菌的克隆基因完全取代了trpC2和trpF基因的功能。
