Mercurio A R, Rhodin J A
J Morphol. 1978 May;156(2):141-55. doi: 10.1002/jmor.1051560203.
This investigation describes the pre-natal morphogenesis of the type I pneumocyte subsequent to its differentiation from pulmonary epithelium. Cells lining subpleural alveolar septa were photographed from serial sections with the electron microscope, and a three-dimensional representation of each cell was obtained by transferring the contours of the cell membranes from montages to transparent plastic sheets which were then spaced to scale and stacked. The results of this study indicate that: The nascent blood-air barrier of a 50-day reconstructed cell was twice as thick as the average definitive barrier; definitive barrier thickness was observed in some areas in a 63-day reconstructed cell; the amorphous component of elastic tissue which appears peripherally in septal connective tissue during pre-natal morphogenesis may be directly juxtaposed to the basal lamina of the alveolar epithelium; the orientation of the cell junction between a pneumocyte and its neighboring cells, as observed in sections of alveolar septa, changes as the contour of the pneumocyte changes from simple abutment to overlapping patterns.
本研究描述了I型肺上皮细胞从肺上皮分化后的产前形态发生过程。用电子显微镜从连续切片中拍摄胸膜下肺泡隔内衬细胞的照片，并通过将细胞膜轮廓从蒙片转移到透明塑料片上，然后按比例间隔并堆叠，获得每个细胞的三维图像。本研究结果表明：50天重构细胞的新生气血屏障厚度是平均最终屏障厚度的两倍；在63天重构细胞的某些区域观察到最终屏障厚度；产前形态发生过程中在隔结缔组织周边出现的弹性组织无定形成分可能直接与肺泡上皮基膜相邻；在肺泡隔切片中观察到，随着肺上皮细胞轮廓从简单邻接模式变为重叠模式，肺上皮细胞与其相邻细胞之间的细胞连接方向发生变化。
