Davey R A, Harvie R M, Cahill E J, Levi J A
Eur J Cancer Clin Oncol. 1984 Jan;20(1):75-9. doi: 10.1016/0277-5379(84)90037-3.
High resolution agarose isoelectric focusing was used to compare the galactosyltransferase isoenzyme patterns of serum from 9 healthy controls with those from 38 patients with either breast, lung, ovarian, stomach or colonic cancer. At least 12 peaks of enzyme activity were found in every sample, the healthy controls having major forms with isoelectric points of 4.74, 4.87, 4.96, 5.16 and 5.23. Thirty patients (79%) had elevated levels of at least one isoenzyme and 23 (61%) had at least 3 isoenzymes elevated compared to only 10 (26%) patients who had elevated total serum galactosyltransferase activity. The isoenzymes which were most often elevated in the cancer patient group had isoelectric points of 4.93, 5.16 and 4.61. One isoenzyme with an isoelectric point of 4.43 was preferentially elevated in patients with ovarian cancer. Those isoenzymes containing little or no sialic acid were rarely elevated in cancer patients. Although no cancer-associated isoenzyme was detected the quantitative differences observed in the cancer patient group were striking.
采用高分辨率琼脂糖等电聚焦法，比较了9名健康对照者与38名乳腺癌、肺癌、卵巢癌、胃癌或结肠癌患者血清中的半乳糖基转移酶同工酶谱。每个样本中至少发现了12个酶活性峰，健康对照者的主要同工酶形式的等电点为4.74、4.87、4.96、5.16和5.23。与仅有10名（26%）血清总半乳糖基转移酶活性升高的患者相比，30名患者（79%）至少有一种同工酶水平升高，23名患者（61%）至少有3种同工酶水平升高。癌症患者组中最常升高的同工酶等电点为4.93、5.16和4.61。一种等电点为4.43的同工酶在卵巢癌患者中优先升高。那些含很少或不含唾液酸的同工酶在癌症患者中很少升高。虽然未检测到与癌症相关的同工酶，但癌症患者组中观察到的定量差异很显著。
