The Ia-1 gene effects T-B cell collaboration for the in vitro antibody response.

The I-A subregion mutant B6.C-H-2bm12 (bm12) and the C57BL/6 parent were used to examine the role of the Ia-1 gene product as a restriction element in the antibody response to sheep red blood cells (SRBC). Using an in vitro culture system, it was shown that cooperation between histocompatible T cells, B cells and macrophages was required to generate a secondary IgM response to SRBC. The alteration in the Ia-1 gene in bm12 altered the ability of bm12 T cells to collaborate with C57BL/6 B cells, but not with C57BL/6 macrophages. Similarly, C57BL/6 T cells could not collaborate with bm12 B cells. The mutation in bm 12 did not affect the ability of T cells to interact with C57Bl/6 macrophages, either in vitro or in vivo, or of bm12 macrophages to interact with C57BL/6T cells. Thus in this system, the Ia-1 gene product restricts T-B cell interaction but not T cell-macrophage interaction.