Williams K A, Vanderwel M, Haslam R J
FEBS Lett. 1984 Jan 23;166(1):13-8. doi: 10.1016/0014-5793(84)80035-6.
NaCl stimulated the adenylate cyclase activities of human and rabbit platelet particulate fractions prepared in the presence of ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N',-tetraacetate, but inhibited the activities of particulate fractions proteolysed by endogenous Ca2+-activated protease or treatment with alpha-chymotrypsin. Studies with other monovalent cations showed that LiCl had weak effects similar to those of NaCl, whereas KCl inhibited the enzyme in both proteolysed and non-proteolysed preparations. The results suggest that NaCl exerts stimulatory and inhibitory effects through different sites. NaCl potentiated and proteolysis greatly reduced the inhibition of platelet adenylate cyclase by 1-O-octadecyl-2-O-acetyl-sn-glyceryl-3-phosphorylcholine (platelet-activating factor).
氯化钠刺激了在乙二醇双（β-氨基乙基醚）-N，N，N'，N'-四乙酸存在下制备的人和兔血小板微粒体部分的腺苷酸环化酶活性，但抑制了由内源性Ca2 +激活的蛋白酶蛋白水解或用α-胰凝乳蛋白酶处理后的微粒体部分的活性。对其他单价阳离子的研究表明，LiCl具有与NaCl相似的微弱作用，而KCl在蛋白水解和未蛋白水解的制剂中均抑制该酶。结果表明，NaCl通过不同位点发挥刺激和抑制作用。NaCl增强作用且蛋白水解大大降低了1-O-十八烷基-2-O-乙酰基-sn-甘油-3-磷酸胆碱（血小板活化因子）对血小板腺苷酸环化酶的抑制作用。
