Massicotte J, Lagacé L, Godbout M, Labrie F
J Endocrinol. 1984 Feb;100(2):133-40. doi: 10.1677/joe.0.1000133.
The incubation of female rat adenohypophysial cells in primary culture with porcine granulosa cell culture medium (GCM) led to the complete inhibition of responses of LH and FSH to LH releasing hormone (LHRH) as well as to the inhibition of spontaneous release of FSH. These effects of GCM suggest the specificity of the 'inhibin'-like activity of this material. Granulosa cell culture medium completely reversed the stimulatory effect of oestradiol-17 beta on the responses of LH and FSH to LHRH, as well as reversing the stimulatory effect of progesterone, oestradiol or a combination of both steroids on the spontaneous release of FSH, while not affecting the spontaneous release of LH. The antioestrogenic effects of progesterone observed on the response of LH to 0.3 nM-LHRH were amplified in the presence of GCM while the stimulatory effects of progesterone, oestradiol or both on the response of FSH to 0.3 nM-LHRH were completely reversed by the medium. Moreover, the presence of GCM led to an additive inhibitory effect with dihydrotestosterone on the response of LH to LHRH while it completely reversed the stimulatory effect of the androgen on spontaneous and LHRH-induced FSH release. The present data show that the presence of porcine granulosa cell 'inhibin' activity can exert marked interactions with sex steroids in the control of gonadotrophin secretion. This 'inhibin' activity reversed all the stimulatory effects and potentiated all the inhibitory effects of sex steroids on gonadotrophin secretion. Although the physiological role of 'inhibin' remains to be defined well, the importance of this activity is clearly demonstrated in anterior pituitary cells in culture.
将原代培养的雌性大鼠腺垂体细胞与猪颗粒细胞培养基（GCM）一起孵育，会导致促黄体生成素（LH）和促卵泡生成素（FSH）对促黄体生成素释放激素（LHRH）的反应完全受到抑制，同时FSH的自发释放也受到抑制。GCM的这些作用表明该物质具有类似“抑制素”活性的特异性。颗粒细胞培养基完全逆转了17β-雌二醇对LH和FSH对LHRH反应的刺激作用，同时也逆转了孕酮、雌二醇或这两种类固醇组合对FSH自发释放的刺激作用，而对LH的自发释放没有影响。在GCM存在的情况下，观察到孕酮对LH对0.3 nM - LHRH反应的抗雌激素作用增强，而孕酮、雌二醇或两者对FSH对0.3 nM - LHRH反应的刺激作用则被该培养基完全逆转。此外，GCM的存在与双氢睾酮对LH对LHRH反应产生相加抑制作用，同时它完全逆转了雄激素对自发和LHRH诱导的FSH释放的刺激作用。目前的数据表明，猪颗粒细胞“抑制素”活性的存在在促性腺激素分泌的控制中可与性类固醇产生显著相互作用。这种“抑制素”活性逆转了性类固醇对促性腺激素分泌的所有刺激作用，并增强了所有抑制作用。尽管“抑制素”的生理作用仍有待明确界定，但这种活性在培养的垂体前叶细胞中的重要性已得到明确证明。
