Pilgrim H, Kumpfmüller A, Teuscher E
Acta Histochem. 1983;73(2):145-50.
The mean relative content of myosin of cells from smooth muscular organs was estimated using immunocytochemical methods after enzymatical tissue disintegration. The cells were classified according to myosin content. Aortas and urinary bladders of newborn rats and chicken amnions were used as models. Cells of a permanent cell strain, being free of smooth muscular myosin served as negative controls. It could be shown, that the content of myosin, estimated by immunocytochemical methods correlates with the state of development of smooth muscular organs.
采用酶解法使组织分散后，运用免疫细胞化学方法对来自平滑肌器官的细胞中肌球蛋白的平均相对含量进行了评估。根据肌球蛋白含量对细胞进行分类。新生大鼠的主动脉和膀胱以及鸡羊膜被用作模型。一种不含平滑肌肌球蛋白的永久细胞系的细胞用作阴性对照。结果表明，通过免疫细胞化学方法评估的肌球蛋白含量与平滑肌器官的发育状态相关。
