Barni S, Gerzeli G, Gervaso M V
Acta Histochem. 1983;73(2):251-7. doi: 10.1016/S0065-1281(83)80034-8.
The PAS-positive material was measured by cytofluorometry in peripheral circulating erythrocytes of 22 species from different classes of Vertebrates. The following parameters were considered: total fluorescence intensity per cell, concentration index, fluorescence fading. The highest variability of concentration indexes was found in Fishes with a minimum in the more active swimmers (Scylliorhinus and Salmo); this fact may be related to a more intense erythropoiesis. The values were more homogeneous in the Amphibians, Reptiles, and Birds, the difference never exceeding 1/2 between the maximum and minimum values. The lowest concentration indexes were found in Mammals, a class with very specialized and enucleated erythrocytes: the data appear rather heterogeneous with a minimum in Macaca. The patterns of fluorescence fading suggest a biochemical homogeneity of the PAS-positive erythrocyte material in the species considered. This fact might indicate the presence of a fundamental biochemical component possibly linked to different structures of the red cells.
通过细胞荧光测定法对来自不同脊椎动物类别的22个物种的外周循环红细胞中的PAS阳性物质进行了测量。考虑了以下参数：每个细胞的总荧光强度、浓度指数、荧光衰减。在鱼类中发现浓度指数的变异性最高，而在活动能力较强的游泳者（鲨鳐和鲑鱼）中最低；这一事实可能与更强烈的红细胞生成有关。两栖动物、爬行动物和鸟类的值更为均匀，最大值和最小值之间的差异从未超过1/2。在哺乳动物中发现了最低的浓度指数，哺乳动物的红细胞非常特殊且无细胞核：数据显得相当不均匀，在猕猴中最低。荧光衰减模式表明在所研究的物种中PAS阳性红细胞物质具有生化同质性。这一事实可能表明存在一种基本的生化成分，可能与红细胞的不同结构有关。
