Ohshima K, Shargill N S, Chan T M, Bray G A
Am J Physiol. 1984 Feb;246(2 Pt 1):E193-7. doi: 10.1152/ajpendo.1984.246.2.E193.
Glucose transport and insulin resistance in the skeletal muscle of the genetically obese (ob/ob) mouse has been studied in animals at 6-7 wk of age using an in situ perfused hindquarter preparation. Rates of glucose uptake and the uptake of radioactively labeled 2-deoxy-D-glucose were lower in muscles from intact ob/ob mice than in those from their lean littermates. Following adrenalectomy the basal rate of glucose or 2-deoxy-D-glucose uptake in the ob/ob mice was restored to normal. The uptake of 2-deoxyglucose was tested at two insulin concentrations. In lean mice, adrenalectomy had no effect on 2-deoxyglucose uptake. Adrenalectomy, however, restored the responsiveness to insulin in the ob/ob mouse to normal. The activity ratio of muscle glycogen synthase was elevated by insulin but the effect was greater in the lean animals with or without adrenal glands than in the obese mouse. Adrenalectomy in the obese mouse did not correct this defect.
利用原位灌注后肢制备技术，对6 - 7周龄的遗传性肥胖（ob/ob）小鼠骨骼肌中的葡萄糖转运和胰岛素抵抗进行了研究。完整ob/ob小鼠肌肉中的葡萄糖摄取率和放射性标记的2-脱氧-D-葡萄糖摄取率低于其瘦同窝小鼠的肌肉。肾上腺切除术后，ob/ob小鼠的基础葡萄糖或2-脱氧-D-葡萄糖摄取率恢复正常。在两种胰岛素浓度下测试了2-脱氧葡萄糖的摄取。在瘦小鼠中，肾上腺切除术对2-脱氧葡萄糖摄取没有影响。然而，肾上腺切除术使ob/ob小鼠对胰岛素的反应恢复正常。胰岛素可提高肌肉糖原合酶的活性比，但无论有无肾上腺，瘦动物中的作用都比肥胖小鼠更大。肥胖小鼠的肾上腺切除术并未纠正这一缺陷。
