Makler A, Fisher M, Murillo O, Laufer N, DeCherney A, Naftolin F
Fertil Steril. 1984 Mar;41(3):428-32. doi: 10.1016/s0015-0282(16)47723-x.
The role of various environmental conditions on sperm motility and their survival was investigated by incubating washed human spermatozoa in various biologic or artificial media and under different atmospheric compositions. When sperm were analyzed by the multiple exposure photography technique for objective motility determination, it was found that none of the investigated biologic media induced any immediate stimulatory effect on the tested spermatozoa. Exchange of seminal fluid between normal and oligoasthenospermic specimens did not reveal any beneficial or harmful effect on sperm motility. Sperm did not survive for more than 24 hours when kept in their original seminal fluid under any circumstances. The most favorable media tested for prolonged sperm survival were pure human umbilical cord serum or Ham's solution containing 0.5% human albumin. However, even in these media sperm did not survive much beyond 24 hours unless incubated in an atmosphere containing 5% CO2 or a triple mixture of 90% N2, 5% O2, and 5% CO2. Only under these circumstances could sperm stay alive, in some cases up to 5 days.
通过将洗涤后的人类精子在各种生物或人工培养基中以及不同的大气成分下进行孵育，研究了各种环境条件对精子活力及其存活的影响。当采用多次曝光摄影技术对精子进行分析以客观测定其活力时，发现所研究的任何生物培养基均未对受试精子产生任何即时刺激作用。正常和少弱精子症标本之间的精液交换未显示出对精子活力有任何有益或有害影响。在任何情况下，精子保存在其原始精液中时存活时间均不超过24小时。经测试，最有利于精子长期存活的培养基是纯人脐带血清或含0.5%人白蛋白的哈姆氏溶液。然而，即使在这些培养基中，除非在含5%二氧化碳的气氛中或90%氮气、5%氧气和5%二氧化碳的三元混合气体中孵育，精子存活时间也不会超过24小时太多。只有在这些情况下精子才能存活，在某些情况下可达5天。
