Speert D P, Eftekhar F, Puterman M L
Infect Immun. 1984 Mar;43(3):1006-11. doi: 10.1128/iai.43.3.1006-1011.1984.
Pseudomonas aeruginosa is the predominant respiratory pathogen in patients with cystic fibrosis, but its mechanism of persisting in pulmonary secretions is poorly understood. We observed that three nonmucoid cystic fibrosis P. aeruginosa strains were phagocytized and one strain resisted phagocytosis by human polymorphonuclear leukocytes in the absence of serum. Phagocytosis was assessed by luminol-enhanced chemiluminescence, inspection of stained smears, bactericidal assay, reduction of nitroblue tetrazolium dye, and electron microscopy. Phagocytosis, determined by visual inspection, occurred at 35 degrees C but not at 4 degrees C. Nonopsonic phagocytosis was inhibited most efficiently by D-mannose, mannose-containing saccharides, and D-fructose. Opsonin-dependent phagocytosis of P. aeruginosa and of zymosan was not markedly inhibited by mannose, suggesting different leukocyte receptors for nonopsonic and opsonic phagocytosis.
铜绿假单胞菌是囊性纤维化患者的主要呼吸道病原体，但其在肺分泌物中持续存在的机制尚不清楚。我们观察到，在无血清条件下，三株非黏液型囊性纤维化铜绿假单胞菌菌株被人类多形核白细胞吞噬，而一株菌株抵抗吞噬。通过鲁米诺增强化学发光、染色涂片检查、杀菌试验、硝基蓝四氮唑染料还原及电子显微镜评估吞噬作用。通过目视检查确定，吞噬作用在35℃时发生，但在4℃时不发生。非调理吞噬作用最有效地被D-甘露糖、含甘露糖的糖类和D-果糖抑制。铜绿假单胞菌和酵母聚糖的调理素依赖性吞噬作用未被甘露糖明显抑制，提示非调理吞噬作用和调理吞噬作用存在不同的白细胞受体。
